15Assassin’s Creed Syndicate - Jacob Frye
This may still be a debated topic amongst fans, but according to the writer for Assassin’s Creed Syndicate, Jacob is canonically bisexual.
The evidence is there. During the course of the game, Jacob eventually teams up with Maxwell Roth, who over the course
of events fall in love with him. Jacob maintains a certain distance throughout, however, at the end of Reclaiming Control of London, Jacob and Maxwell share a kiss as Jacob painfully ends his life.
You may think that kiss was just a sign of Roth’s madness, but the lead writer confirmed it was because he was madly in love with Jacob. He goes on to confirm Jacob’s bi-ness by saying he has to “figure himself out” after the tragic death.